Coding
HAO

Part:BBa_K4354005:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-22)


hao, partial [Nitrosomonas europaea]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 364
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 262


Design Notes

By cloning AMO,HAO,NAR,NAR,NOR,NOS to the Bacillus subtilis, We want to make it an engineering strain that could remove the nitrogenous compounds in the waste water.


Source

We search its sequence from NCBI(AEL00662) and synthesize it.

References

1.Song Qin, Xu Lei.Advancement of Enzymology of Heterotrophic Nitrification.BIOTECHNOLOGY BULLETIN.DOI:10.13560/j.cnki.biotech.bull.1985.2008.05.009 2.Xu Tao.METABOLIC CHARACTERISTICS OF HETEROTROPHIC NITRIFYING-AEROBIC DENITRIFYING STRAIN DIAPHOROBACTER SP.PD-7.Taiyuan University of Technology in China.2015 May10.