Coding
HAO
Part:BBa_K4354005:Design
Designed by: Java Yao Group: iGEM22_GXU-China (2022-07-22)
hao, partial [Nitrosomonas europaea]
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 364
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 364
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 364
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 364
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 364
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 262
Design Notes
By cloning AMO,HAO,NAR,NAR,NOR,NOS to the Bacillus subtilis, We want to make it an engineering strain that could remove the nitrogenous compounds in the waste water.
Source
We search its sequence from NCBI(AEL00662) and synthesize it.
References
1.Song Qin, Xu Lei.Advancement of Enzymology of Heterotrophic Nitrification.BIOTECHNOLOGY BULLETIN.DOI:10.13560/j.cnki.biotech.bull.1985.2008.05.009 2.Xu Tao.METABOLIC CHARACTERISTICS OF HETEROTROPHIC NITRIFYING-AEROBIC DENITRIFYING STRAIN DIAPHOROBACTER SP.PD-7.Taiyuan University of Technology in China.2015 May10.